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•4-gas-mix For stem cell & mammalian cell cultures |
•3-gas-mix For anaerobic fermentation |
•O2-enrichment For microbial systems & biofuel development |
The MINIFOR bioreactor and fermentor system is used for growing and testing bacteria, yeast, fungi, mammalian cells and stem cells.
MINI-4-GAS is a module for MINIFOR that allows a completely automatic gas-mix.
The MINI-4-GAS consists of MASSFLOWs and PC-Software.
MASSFLOWS will be measuring and regulating the gas-flow of the desired gasses.
It could be controlled remotely using LAMBDA SIAM PC control software extension.
The LAMBDA gas-mixing module allows a flexible mass flow controlled supply of different gases with individual gas flow paths. |
The gassing system automatically mixes up to 4 gases for mammalian cell systems as well as microbial applications at bench scale. |
The automatically controlled aeration system provides an advanced gas diffusion by sparging and / or headspace gassing. |
The extension of LAMBDA SIAM PC control software allows a complete automatic gas-mix control for up to 4 MINIFOR parallel-reactors (4 x 4 gas flow controller) |
MINIFOR bioreactor * Submerge (up to 5 l/min) * Headspace (up to 20 l/min) Out-of-the-box start with the 4-gas connection kit; RS-232/RS-485 interface. Porous self-cleaning sparger; pH; pO2; (REDOX, pCO2); Temperature; Agitation; Peristaltic pumps. Anaerobic / Aerobic fermentation; Mammalian cell culture, Long term culture; Batch, Fedbatch, Continuous, Chemostat and Perfusion |
SIAM PC control software * Handles up to 4 bioreactor units with their individual 4-gas controllers. Process control PC-software; Pre-configured & Installed on Laptop or Notebook; Data storage & Plotting; Data acquisition, calculations & gas transfer rates; trend graphs display; extended visualization; |
MASSFLOW * Free selection of gas types: Air, Oxygen, Carbon dioxide, Nitrogen for high precison; (optional: Methane, Carbon monoxide, ...) * Individual flow rates per gas: 0–5 l/min (MASSFLOW 5000), Stand-alone & Programmable Extremely user friendly; Durable & Reliable; Electrical supply by MINIFOR (optional: stand-alone) |
Which gases do your cells need?
Example 4-GAS Mixing for mammalian cells: pO2 control & O2 enrichmentpH control by CO2Constant gas flow
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Build now your own automatic gas-mixing module!
MINI-4-GAS is as easy to interlock as plastic bricks:
The bricks of MINI-4-GAS are LAMBDA MASSFLOWS. Each MASSFLOW will control one gas in your gas-mixture. You can choose two, three or four gases for your gas-mix.
The MASSFLOWS will dose and measure the gas flow rates accoring your set-points (flow rate / pH / pO2 / pCO2…)
The MINI-4-GAS software (a SIAM extension) is the brain of the gas-mix. The PC-software controls, visualizes and saves all gas-flow data.
The MINIFOR bioreactor dissolve the gases by micro-sparger, surface-aeration and excellent stirring with FISH-TAIL – a most efficient gas-uptake under minimized shearing forces.
The SIAM extension
Real-time trend graphs
Vizualization of the entire process & data storage. |
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Individual PID controller for each gas
Easy set-point adjustment Low & High flow rate limits for each gas |
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pO2 control with Air & Oxygen (O2-enrichment) pH control with Carbon dioxide Optional: constant total gas flow (exclusive flow) |
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Compared to most bio-transformations with quick growing yeast or bacteria, mammalian cell cultures by their low specific growth rates have to be cultured for weeks or even months for satisfying the product yields.
MINIFOR bioreactor offers you the “Lambda Easy Sterility” concept and an excellent peristaltic pumps specially constructed for the long-term cell cultures. The MINIFOR with the 4-gas-mixing-module maintains the optimal physiologic conditions for your mammalian cells and the stem cell cultures using the modern control strategies.
Controlling the critical process parameters will help you to optimize the growth rates as well as to achieve the higher titres during protein processing and hormone production.
LAMBDA Item N° |
Item Description |
Amount for |
800012 |
Additional regulated gas flow |
n |
800013 |
Additional regulated gas flow |
4-n |
4803 |
PUMP-FLOW INTEGRATOR for Massflow |
(4) optional |
4816 |
RS-485 interface |
4 |
4810 |
Pump remote control (analog and digital) cable for LAMBDA MASSFLOW (8 poles) |
4 |
800202 |
Quadruple plug box (power and RS-connection for up to 4 LAMBDA laboratory instruments) |
1 |
800603 |
SIAM industrial automation software |
1 |
800603-g |
Gas controller software module |
1 |
800602 |
PC connection kit with RS 485 to RS 232 converter |
1 |
800604 |
quality brand laptop with Windows 7 operating system |
1 |
4819-USB |
USB to RS-232 converter |
1 |